PD Dr. Uwe Müller

PD Dr. Uwe Müller

Research Fellow

Immunologie
Biotechnologisch-Biomedizinisches Zentrum
Deutscher Platz 5, Room 1.304
04103 Leipzig

Phone: +49 341 97-31224
Fax: +49 341 97-31229

Abstract

Uwe Müller is a biologist and work since 2001 at the University of Leipzig at the faculty of veterinary medicine under supervision of Prof. Gottfried Alber in the field of infectious immunology. He is interested in the interaction of Cryptococcus neoformans, a humanpathogenic fungus, with the immune system of the lung. Before his recent interest in the role of alveolar epithelial cells in pulmonary cryptococcosis, he had a grant of the DFG, focussing on the Th2 response in cryptococcal infection. Besides his scientific interests he is a lecturer for immunology, laboratory animal science, and genetics.

His methodological portfolio includes RT PCR, western blotting and for analyses of cells flow cytometric approaches and ELISA techniques. To measure barrier functions of cells he uses electrical procedure.

Professional career

  • since 04/2003
    Scientific position, Institute of Immunology (Veterinary faculty, University of Leipzig)
  • 04/2013
    Habilitation in Immunology (Veterinary faculty, University of Leipzig)
  • 12/2001 - 03/2003
    Assistance position, Institute of Immunology (Veterinary faculty, University of Leipzig)
  • 07/2001 - 11/2001
    Post Doc position, Institute of Immunology (Veterinary faculty, University of Leipzig)
  • 06/1998 - 05/2001
    Doctoral thesis in Biology, work group Parasitology (Ruhr University of Bochum) at the Max Planck Institute of Immunobiology, Freiburg

Education

  • 06/1997 - 05/1998
    Diploma thesis in Biology, work group Parasitology (Ruhr University of Bochum) at the Max Planck Institute of Immunobiology, Freiburg
  • 10/1992 - 05/1997
    Studies of Biology a the Ruhr University of Bochum

In his diploma and doctoral theses Uwe Müller focussed on the infectious immunological question of interactions of the intracellular parasite Trypanosoma cruzi with the host immune system at the Max Planck Institute of Immunobiology. Subsequent he switched his field of interest from parasites to fungal pathogens, therefore he moved to the Institute of Immunology in the Veterinarian Faculty of the University of Leipzig. Under the supervision of Prof. Alber he examined the role of allergy-related cytokines and cytokine receptors in pulmonary cryptococcosis. Concomitant with this work he raised funds from the DFG to answer question to characterize cells involved in immunopathology in pulmonary cryptoccococsis. In a recent project he focussed on the role of lung epithelial cells in cryptococcosis, especially barrier function and modulation of the immune response. For this work he established in vitro models.


Methods:

  • PCR, RT-PCR
  • SDS-PAGE/Westerblot
  • ELISA
  • FACS analysis and sorting
  • In vitro/vivo models
  • Role of early IL-4 in pulmonary cryptococcosis
    Müller, Uwe
    Duration: 05/2011 - 08/2015
    Funded by: DFG Deutsche Forschungsgemeinschaft
    Involved organisational units of Leipzig University: Molekulare Pathogenese; Biotechnologisch-Biomedizinisches Zentrum (BBZ)
    show details
  • In vitro system for characterizing the allergic potential of human and animal pathogenic fungi
    Müller, Uwe
    Duration: 09/2019 - 03/2021
    Funded by: BMBF Bundesministerium für Bildung und Forschung
    Involved organisational units of Leipzig University: Immunologie; Biotechnologisch-Biomedizinisches Zentrum (BBZ)
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more projects

  • Piehler, D.; Eschke, M.; Schulze-Richter, B.; Protschka, M.; Müller, U.; Grahnert, A.; Richter, T.; Heyen, L.; Köhler, G.; Brombacher, F.; Alber, G.
    The IL-33 receptor (ST2) regulates early IL-13 production in fungus-induced allergic airway inflammation
    Mucosal Immunology. 2016. 9 (4). pp. 937-949
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  • Müller, U.; Stenzel, W.; Piehler, D.; Grahnert, A.; Protschka, M.; Köhler, G.; Frey, O.; Held, J.; Richter, T.; Eschke, M.; Kamradt, T.; Brombacher, F.; Alber, G.
    The role of macrophages in IL-4 receptor (IL-4R)-dependent pathology in pulmonary cryptococcosis
    MYCOSES. 2015. 58 (S3). pp. 65-66
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  • Müller, U.; Stenzel, W.; Piehler, D.; Grahnert, A.; Protschka, M.; Köhler, G.; Frey, O.; Held, J.; Richter, T.; Eschke, M.; Kamradt, T.; Brombacher, F.; Alber, G.
    Abrogation Of Il-4 Receptor-Alpha-Dependent Alternatively Activated Macrophages Is Sufficient To Confer Resistance Against Pulmonary Cryptococcosis Despite An Ongoing T(H)2 Response
    International immunology. 2013. 25 (8). pp. 459-470
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  • Müller, U.; Piehler, D.; Stenzel, W.; Köhler, G.; Frey, O.; Held, J.; Grahnert, A.; Richter, T.; Eschke, M.; Kamradt, T.; Brombacher, F.; Alber, G.
    Lack Of Il-4 Receptor Expression On T Helper Cells Reduces T Helper 2 Cell Polyfunctionality And Confers Resistance In Allergic Bronchopulmonary Mycosis
    Mucosal immunology. 2012. 5 (3). pp. 299-310
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  • Stenzel, W.; Müller, U.; Köhler, G.; Heppner, F. L.; Blessing, M.; McKenzie, A. N. J.; Brombacher, F.; Alber, G.
    Il-4/Il-13-Dependent Alternative Activation Of Macrophages But Not Microglial Cells Is Associated With Uncontrolled Cerebral Cryptococcosis
    The American journal of pathology. 2009. 174 (2). pp. 486-496
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more publications

  • Molekulare Pathogenese; Medizinische Fakultät und Universitätsklinikum AöR; Biotechnologisch-Biomedizinisches Zentrum (BBZ)
    Pulmologische Untersuchungen während der Kryptokokkose
    Start Date of Collaboration: 01/12/2016
    Involved persons: Müller, Uwe; Polte, Tobias
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  • Molekulare Pathogenese; Institut für Parasitologie; Biotechnologisch-Biomedizinisches Zentrum (BBZ)
    Charakterisierung von Kryptosporidienstadien mittels Durchflusszytometrie
    Start Date of Collaboration: 01/01/2016
    Involved persons: Müller, Uwe; Daugschies, Arwid; Lendner, Matthias
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  • Molekulare Pathogenese; Medizinische Fakultät und Universitätsklinikum AöR; Institut für Immunologie; Biotechnologisch-Biomedizinisches Zentrum (BBZ)
    Mikroskopische Untersuchungen von Alveolarepithel während der pulmonalen Kryptokokkose
    Start Date of Collaboration: 01/01/2015
    Involved persons: Alber, Gottfried; Gericke, Martin; Heyen, Laura; Piehler, Daniel; Müller, Uwe
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more cooperations

Immunology, laboratory animal science, and genetics are the main topics of Uwe Müller's lectures. Since 2001 Uwe Müller participates in the lectures, required electives, and trainings in immunology and laboratory animal science. In 2014 he resumed the genetics lectures. Together with Prof. Alber and Dr. Eschke Uwe Müller is responsible for the teachings of the Institute of Immunology at the Veterinary faculty.

Recently he is involved in the following courses

Immunology

  • Focus Erkennen, Verstehen, Behandeln (5/6th sem.): humoral immunological diagnostics (2h)
  • Focus Blut (8th sem.): canine and feline blood types (1h)
  • Focus Geflügel (8th sem.): The avian immune system & vaccination strategies (2h)
  • Focus Zelle (2nd sem.): cell cycle, from gene to protein (3h)


Laboratory animal science

  • Laboratory animal science (5th sem.): laboratory mouse, pig (2-3h)


Animal breeding and genetics

  • Genetics (2nd/3rd sem.): (i) classical and molecular genetics, (ii) population genetics and evolution, (iii) pathogenetics (42h)


Exams

Immunology

  • written examination (mandatory for all students)


Laboratory animal science

  • written examination (optional)


Animal breeding and genetics

  • written examination (mandatory for all students), 3. try oral examination


  • Zoology (part: evolution, immunology)

    Aspects and forces of evolution will be presented in this lecture in 3 lessons. In 5 lessions immunological aspects, i.a. innate immunity and immune evasion mechanisms will be in focus.

  • Animal breeding and Genetics

    In this lecture different aspects of genetic will be in focus, (i) classic and molecular genetics, (ii) population genetics and evolution, and (iii) pathogenetics. In total the lecture comprises 42 lessons in the 2nd and 3rd term.

  • Laboratory animals

    Experts in their field present different species of laboratory animals and applications in which they are used. Beside animal models also aspects of ethics and alternative methods will be demonstrated.